Title: Modelling and measurements of fibrinogen adsorption on positively charged microspheres
Author(s):

	P. Zeliszewska ( J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, 30-239 Cracow, Poland ) ,
	A. Bratek-Skicki ( J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, 30-239 Cracow, Poland ) ,
	Z. Adamczyk ( J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, 30-239 Cracow, Poland ) ,
	M. Ciesla ( M. Smoluchowski Institute of Physics, Jagiellonian University, Lojasiewicza 11, 30-348 Cracow, Poland )

Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10^-3 - 0.15 M. The maximum coverage of fibrinogen on latex particles was precisely determined by the AFM method. Unexpectedly, at pH = 3.5, where both fibrinogen molecule and the latex particles were positively charged, the maximum coverage varied between 0.9 mg m^-2 and 1.1 mg m^-2 for 10^-2 and 0.15 M NaCl, respectively. On the other hand, at pH = 9.7, the maximum coverage of fibrinogen was larger, varying between 1.8 mg m^-2 and 3.4 mg m^-2 for 10^-2 and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations.

Key words: fibrinogen adsorption, positively charged microspheres
PACS: 82.70.Dd, 87.15A, 87.14.E

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